Objective:To study factors affecting Amplified Fragment Length Polymorphism (AFLP) in A ster tataricus L.
目的:探讨影响紫菀扩增片断长度多态性(Amplified Fragment Length Polymorphism,AFLP)的各种因素,建立并优化紫菀AFLP反应体系,为研究紫菀分子品质性状的技术平台奠定基础。
Amplified Fragment length Polymorphism(AFLP),a new molecular marker technique, has been applied in wheat genetic diversity study, germplasm fingerprinting and characterization, wheat genetic linkage map construction,(identification ) of molecular markers tightly linked to the gene, molecular-markers assistant selection and so on.
AFLP(Amp lified Fragm ent length Polymorph ism扩增性片段长度多态性)是一种新的DNA分子标记,近年来已应用于小麦及小麦种质资源遗传多样性、种质指纹图谱构建及遗传材料鉴定、小麦遗传连锁图谱的构建、目的基因的分子定位、分子标记辅助选择等方面的研究。
Some research methods about plant functional genomics,such as AFLP,SAGE,transposable element,biochip,Bioinformatics were introduced as well as the prospects for their development.
主要阐述了植物功能基因组学的研究方法,如扩增性片段长度多态性、基因表达系列分析技术、转座因子、生物芯片、生物信息学等,并对其发展前景加以展望。
