Screening of Aspergillus strains producing β-fructofuranosidase and fermentation study;
产β-呋喃果糖苷酶菌株的筛选及发酵条件初探
Effect of composite inhibitor A、B on the activity of β-fructosyltransferase(FTS) and β-fructofuranosidase(FFS)from Aspergillus oryzae GX0011 was studied.
研究了复合抑制剂A、B对β-呋喃果糖苷酶(FFS)与β-果糖基转移酶(FTS)活性的影响。
A β-fructofuranosidase gene of 1 917 bp was cloned from Aspergillus japonicus,and the ORF sequence predicted a protein of 638 amino acids.
通过反转录PCR法成功地扩增出日本曲霉β-呋喃果糖苷酶基因,基因片断长度为1 917bp,编码638个氨基酸。
Selection of condition and bacteria species for producing β-Fructofuranosidase;
β-呋喃果糖苷酶产生菌的筛选及产酶条件初探
Lactosucrose was produced byβ-Fructofuranosidase from Arthrobacter sp.
利用自制β-呋喃果糖苷酶合成低聚乳果糖,温度为42℃,pH为70,酶添加量为05mL,底物浓度为20%时,经十几个小时的反应,获得低聚乳果糖含量约30%。
The properties of β fructofuranosidase (1) from a fructooligosaccharides producing strain, Aspergillus niger SIPI 602, were investigated.
研究了低聚果糖产生菌黑曲霉 (Aspergillus niger) SIPI-60 2的β-呋喃果糖苷酶 (1)的性质。
Purified β fructosyltransferase (1) and β fructofuranosidase (2) from a fructooligosaccharides producing strain, Aspergillus niger SIPI 602, showed only one band on SDS polyacr ylamide gel electrophoresis (SDS PAGE).
用球磨机 Dyno- Mill破碎低聚果糖产生菌黑曲霉 (Aspergillus niger) SIPI- 6 0 2的菌丝体 ,其离心上清液经硫酸铵分级沉淀、Octyl- Sepharose CL - 4 B疏水层析、Q- Sepharose Fast Flow阴离子交换层析、Sephacryl S- 30 0凝胶过滤等步骤得到了在 SDS-聚丙烯酰胺凝胶电泳上均呈单带的两种酶 :β-果糖基转移酶与β-呋喃果糖苷酶。
