Cloning Expression and Purification of Heat Shock Protein HSP60 from Hyperthermophilic Archaeon Pyrococcus furiosus
极端嗜热菌Pyrococcus furiosus热休克蛋白HSP60的克隆表达和纯化
HSPs from hyperthermophilic archaea are concise only with HSP60, sHSP, prefoldin and AAA+proteins, but without HSP100s, HSP90s, HSP70 (DnaK), HSP40 (DnaJ) and GrpE which are common in mesophilic or thermophilic archaea.
随着生物工程产业对于耐高温酶和菌体的需求,极端嗜热古菌热休克蛋白(Heatshock proteins,HSPs)的研究更受重视,其热休克蛋白体系非常简洁,不含HSP100s和HSP90s,就是HSP70(DnaK)、HSP40(DnaJ)和GrpE等嗜温古菌可能含有的在极端嗜热古菌中几乎不含有,即仅包括HSP60、sHSP、prefoldin和AAA+蛋白4大类,因此对其结构、功能和作用机制的研究在理论和实践上都特别有意义。
