Preparation of anti-P21-activated kinase 5 polyclonal antibody and its application in dental germ cells;
抗P-21激活的磷酸化蛋白激酶5多克隆抗体的制备及其在牙胚细胞研究中的应用
Objective To explore the essential conditions of the normal tooth germ cells culture in order to use these cultured cells as the seeding cell in the tissue-engineering of tooth .
目的 摸索正常胎鼠牙胚细胞离体培养所需条件 ,为进一步将其作为牙组织工程种子细胞奠定基础。
pSEAP2-control and pAB-Sig-AP-SV40 were transfected into chick embryo cells and MCF-7 cells.
pSEAP2-control和pAB-Sig-AP-SV40质粒转染鸡胚细胞和乳腺癌细胞(MCF-7),转染48h后以对硝基苯磷酸钠(pNPP)为底物检测PLAP的活性,结果pSEAP2-control在鸡胚细胞和MCF-7细胞中表达PLAP,pAB-Sig-AP-SV40在鸡胚细胞中不表达,而在MCF-7细胞中表达。
Thereafer,to made embryo specimens and abserve the blastocyst rates,the numbers of blastomere and mitotic index of embryos.
1μmol/L),发育至囊胚阶段,制作胚胎标本,观察其囊胚发育率、胚细胞数和分裂相数。
The expression of MFH 1 induced by bone morphogenetic protein 2 (BMP 2) in myoblasts C2C12 was examined by Western blot and Northern blot analysis.
为了研究中胚叶叉头 1(MFH 1)基因在骨骼形成和细胞分化中的作用 ,利用基因重组、杂交瘤技术制作MFH 1单克隆抗体 ,利用蛋白质印迹和RNA印迹分析观察了骨成形蛋白 2 (BMP 2 )诱导小鼠肌胚细胞C2C12表达MFH 1、产生碱性磷酸酶和骨钙蛋白 。
