Effects of feed velocity, operational pressure and enzyme activity of xylanases on ultrafiltration separation of endo-β-xylanase and β-xylosidase were investigated.
研究了进料速度、操作压力、木聚糖酶活大小对内切-β-木聚糖酶和β-木糖苷酶超滤分离的影响。
To study the construction of yeast bioengineering strain which can degrade cellulosic waste, an endo β glucanase Ⅲ(EGⅢ) cDNA gene of Trichoderma viride AS3 3711 was isolated with RT PCR protocol.
为研究构建可降解纤维类固体废弃物的工程菌 ,采用RT PCR方法克隆到绿色木霉 (Trichodermaviride)AS3 371 1的葡聚糖内切酶Ⅲ (EGⅢ )的cDNA基因 ,测序后构建到酿酒酵母 (Saccharomycescerevisiae)诱导型表达载体pYES2上 ,用正交实验对超声波辅助酵母转化系统进行了优化 。
